10.25378/janelia.5613145.v3 Nelson Spruston Nelson Spruston Dezhe Jin Dezhe Jin Ting Zhao Ting Zhao David Hunt David Hunt Rachel Pearcy Rachel Pearcy Ching-Lung Hsu Ching-Lung Hsu ShuTu: Video tutorial Janelia Research Campus 2017 Neuronal morphology Neuroscience 2017-11-27 18:57:08 Media https://janelia.figshare.com/articles/media/ShuTu_an_Open-Source_Software_for_Neuron_Reconstruction_Video_Tutorial/5613145 <p>Here we provide a video tutorial for using the ShuTu software platform.</p><p>ShuTu (Chinese for "dendrite") is a software platform for semi-automated reconstruction of neuronal morphology. It is designed for neurons stained following patch-clamp recording and biocytin filling/staining. With an additional preprocessing step, it can also handle fluorescence images from confocal stacks.</p><p>Even when there is only a single neuron in the imaged volume, automated reconstruction is tricky, because of background. Thus, ShuTu was developed to facilitate a two-step process: automated reconstruction, followed by manual correction. The software uses sophisticated algorithms to perform the automated reconstruction, as well as a convenient user interface to facilitate efficient completion of the reconstruction via manual annotation.</p><div><br></div>