10.25378/janelia.5623621.v4
Nelson Spruston
Nelson
Spruston
David Hunt
David
Hunt
Dezhe Jin
Dezhe
Jin
Ting Zhao
Ting
Zhao
Rachel Pearcy
Rachel
Pearcy
Ching-Lung Hsu
Ching-Lung
Hsu
ShuTu: Mouse Purkinje cell confocal images 63X
Janelia Research Campus
2017
Dendritic morphology
Neuroscience
2017-11-27 19:15:25
Dataset
https://janelia.figshare.com/articles/dataset/ShuTu_an_Open-Source_Software_for_Neuron_Reconstruction_Mouse_Purkinje_Cell_confocal_images_63X/5623621
<p>Here we provide images of the mouse Purkinje cell (63X) for practicing reconstruction using the ShuTu software platform. The cell was imaged by David Hunt in the Spruston Lab. </p><p>ShuTu (Chinese for "dendrite") is a software platform for semi-automated reconstruction of neuronal morphology. It is designed for neurons stained following patch-clamp recording and biocytin filling/staining. With an additional preprocessing step, it can also handle fluorescence images from confocal stacks.</p>
<p>Even when there is only a single neuron in the imaged volume, automated reconstruction is tricky, because of background. Thus, ShuTu was developed to facilitate a two-step process: automated reconstruction, followed by manual correction. The software uses sophisticated algorithms to perform the automated reconstruction, as well as a convenient user interface to facilitate efficient completion of the reconstruction via manual annotation.</p>
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