2P calcium imaging from compass neurons of tethered flies walking on a ball in darkness

2P calcium imaging data from EPG (compass) neurons of the central complex of tethered flies walking in darkness for the following paper:

Noorman M, Hulse BK, Jayaraman V, Romani S, Hermundstad AM (2024). Maintaining and updating accurate internal representations of continuous variables with a handful of neurons.

Data file for flies walking in darkness:

• NoormanEtAlFlyWalkingInDarkness2PBehData.mat: preprocessed 2P calcium imaging and behavior data. Calcium imaging performed at 10 Hz; 20-minute trials; 5 trials/fly.

File organization:

• The file contains MATLAB data structures:
  FDB: Metadata for flies used in the experiment
  TDB: Information about 5 trials (A-E) /fly
  DarkData: structure with multiple data fields
• • ROI_Data_Dfof: [32×12000×50 double]: Time series of EPG population GCaMP activity for each of 32 user-defined wedges of the ellipsoid body (EB)
  • FlyRotate_w2p: [50×12000 double]: time series of the fly's rotational velocity on the ball
  • FlyForward_w2p: [50×12000 double]: time series of the fly's forward velocity
  • Fly: [50×1 double]: Fly ID (used internally)
  • TwoP_Times: [50×12000 double]: time stamps corresponding to two-photon data acquisition
  • FlyPosition_w2p_aligned: [50×12000 double]:
  • WedgeCenters: [32×1 double]: centers of ROIs for analysis of imaging data in the EB
  • BumpPosition: [50×12000 double]: phase of the population vector average within the EB
  • BumpR: [50×12000 double]: amplitude of the population vector average
  • BumpPeak: [50×12000 double]: bump amplitude
  • TwoP_Fs: [50×1 double]: sampling frequency

Associated code:

• Code for all analyses and plotting is available at:
  https://github.com/HermundstadLab/DiscreteRingAttractor