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Isotropic 3D electron microscopy data of isolated murine pancreatic islets treated with low glucose (jrc_mus-pancreas-2)
Pancreatic islets (or Islets of Langerhans) are micro-organs consisting mainly of beta, alpha, delta, polypeptide cells and endothelial cells. Beta cells are the majority of the islet cells. They secrete insulin, which is stored in secretory granules (SGs), in order to maintain blood glucose homeostasis. Beta cells within the islet are heterogeneous in their response to glucose. Furthermore, not all insulin SGs within beta cells have the same likelihood of being released. Large-scale high-resolution FIB-SEM enables biologists to investigate the ultrastructural differences between beta cells within an islet as well as features that require higher resolution such as ribosomes and the cytoskeleton.
Sample: Wild-type mouse pancreatic islets treated with low glucose.
Protocol: High-pressure freezing and freeze-substitution with 2% OsO₄, 1% UA, 0.5% GA, 5% H₂O in acetone with 1% methanol, followed by 0.2% TCH in 80% methanol at RT, 2% OsO₄ in acetone at RT, 1% UA in acetone + 10% methanol.
Contributions: Sample provided by Andreas Mueller and Michele Solimena (Paul Langerhans Institute, Dresden), prepared for imaging by Song Pang (HHMI/Janelia), with imaging and post-processing by C. Shan Xu (HHMI/Janelia).
Dataset ID: jrc_mus-pancreas-2
Final voxel size (nm): 4.00 x 4.00 x 4.00 (X, Y, Z)
Dimensions (µm): 20 x 20 x 30 (X, Y, Z)
Acquisition date: 2019-02-16
Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-pancreas-2
Publication: Mueller et al., 3D FIB-SEM reconstruction of microtubule–organelle interaction in whole primary mouse β cells, JCB (2020), 10.1083/jcb.202010039.