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Isotropic 3D electron microscopy data of isolated murine pancreatic islets treated with low glucose (jrc_mus-pancreas-3)
Pancreatic islets (or Islets of Langerhans) are micro-organs consisting mainly of beta, alpha, delta, polypeptide cells and endothelial cells. Beta cells are the majority of the islet cells. They secrete insulin, which is stored in secretory granules (SGs), in order to maintain blood glucose homeostasis. Beta cells within the islet are heterogeneous in their response to glucose. Furthermore, not all insulin SGs within beta cells have the same likelihood of being released. Large-scale high-resolution FIB-SEM enables biologists to investigate the ultrastructural differences between beta cells within an islet as well as features that require higher resolution such as ribosomes and the cytoskeleton.
Sample: Wild-type mouse pancreatic islets treated with low glucose.
Protocol: High-pressure freezing and freeze-substitution with 1% OsO₄ and 0.1% UA in acetone additionally containing 1% H2O for membrane contrast. Samples were immersed in the cocktail for 5 hours at −90 °C followed by raising the temperature to 0 °C over a time course of 18 hours. Then, samples were washed twice in 100% dry acetone for 1 hour and the temperature was raised to room temperature followed by embedding in Durcupan.
Contributions: Sample provided by Andreas Mueller and Michele Solimena (Paul Langerhans Institute, Dresden), prepared for imaging by Song Pang (HHMI/Janelia), with imaging and post-processing by C. Shan Xu (HHMI/Janelia).
Dataset ID: jrc_mus-pancreas-3
Final voxel size (nm): 4.00 x 4.00 x 4.00 (X, Y, Z)
Dimensions (µm): 20 x 20 x 8 (X, Y, Z)
Acquisition date: 2018-07-07
Dataset URL:Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-pancreas-3