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Isotropic 3D electron microscopy reference data of mouse choroid plexus (jrc_choroid-plexus-2)

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posted on 12.11.2020, 23:53 by C. Shan Xu, Song Pang, Davis Bennett, Michael E. Coulter, Christopher A. Walsh, Harald Hess

Mouse choroid plexus epithelial cells, a primary source of the growth factor conic hedgehog in embryonic mouse brain. Here we present a typical 3D FIB-SEM data set of mouse choroid plexus at 8-nm isotropic voxel sampling. It reveals accumulation of multivesicular bodies near the epithelial cell ventricular surface.

Sample: Mouse choroid plexus

Protocol: Postfixed in 1.0% osmium tetroxide in 0.1M cacodylate buffer (pH 7.4) for 1 hour at room temperature. Following postfixation, the samples were rinsed with buffer, dehydrated through a graded series of ethanol, and embedded in durcupan.

Contributions: Sample provided by Michael Coulter & Christopher Walsh (HHMI/Harvard), prepared for imaging by Song Pang (HHMI/Janelia), with imaging by Song Pang (HHMI/Janelia) and C. Shan Xu (HHMI/Janelia), and post-processing by C. Shan Xu (HHMI/Janelia).

Dataset ID: jrc_choroid-plexus-2

Final voxel size (nm): 8.0 x 8.0 x 8.0 (X, Y, Z)

Dimensions (µm): 29 x 34 x 5 (X, Y, Z)

Acquisition date: 2016-06-14

Dataset URL: https://data.janelia.org/1z1FU

Visualization Website: https://openorganelle.janelia.org/datasets/jrc_choroid-plexus-2

Publication: Coulter et al., 2018 (10.1016/j.celrep.2018.06.100), Xu et al., 2017 (10.7554/eLife.25916)

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