Manual nuclei segmentations in near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of A431 epithelial cells (aic_desmosome-3)

dataset

posted on 2024-09-16, 17:26 authored by CellMap Project TeamCellMap Project Team, David Ackerman, Emma Avetissian, Alyson Petruncio, Rebecca VorimoRebecca Vorimo, Yurii Zubov

Annotation description: Dense segmentations of nuclei in aic_desmosome-3 using Amira-Avizo (Thermo Fisher Scientific) and "Using Amira to manually segment organelles in vEM for machine learning V.3" protocol.

Annotation protocol: Using Amira to manually segment organelles in vEM for machine learning V.3 (http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3)

Software: Amira 3D 2021.1, Classic Segmentation Workroom

Classes annotated: Nuclei

Source (EM) dataset ID: aic_desmosome-3

Source (EM) voxel size (nm): 4 x 4 x 4 (X, Y, Z)

Source (EM) data dimensions (µm): 14.9 x 12.9 x 48.4 (X, Y, Z)

Source (EM) DOI: https://doi.org/10.25378/janelia.22675078

Dataset URL: s3://janelia-cosem-datasets/aic_desmosome-3/aic_desmosome-3.zarr/recon-1/labels/inference/segmentations/nuc/

Visualization website: https://openorganelle.janelia.org/datasets/aic_desmosome-3