Manual nuclei segmentations in near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of COS-7 cell overexpressing ER and mitochondria markers for CLEM (jrc_cos7-11)

Annotation description: Dense segmentations of nuclei in jrc_cos7-11 using Amira-Avizo (Thermo Fisher Scientific) and "Using Amira to manually segment organelles in vEM for machine learning V.3" protocol.

Annotation protocol: Using Amira to manually segment organelles in vEM for machine learning V.3 (http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3)

Software: Amira 3D 2021.1, Classic Segmentation Workroom

Classes annotated: Nuclei

Source (EM) dataset ID: jrc_cos7-11

Source (EM) voxel size (nm): 8.0 x 8.0 x 8.0 (X, Y, Z)

Source (EM) data dimensions (µm): 70 x 8 x 100 (X, Y, Z)

Source (EM) DOI: https://doi.org/10.25378/janelia.13123385

Dataset URL: s3://janelia-cosem-datasets/jrc_cos7-11/jrc_cos7-11.zarr/recon-1/labels/inference/segmentations/nuc/

Visualization website: https://openorganelle.janelia.org/datasets/jrc_cos7-11