Manual nuclei segmentations in near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of Killer T-Cell attacking cancer cell (jrc_ctl-id8-1)

<p dir="ltr"><b>Annotation description</b>: Dense segmentations of nuclei in jrc_ctl-id8-1 using Amira-Avizo (Thermo Fisher Scientific) and "Using Amira to manually segment organelles in vEM for machine learning V.3" protocol.</p><p dir="ltr"><b>Annotation protocol</b>: Using Amira to manually segment organelles in vEM for machine learning V.3 (<a href="http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3" target="_blank"><u>http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3</u></a>)</p><p dir="ltr"><b>Software</b>: Amira 3D 2021.1, Classic Segmentation Workroom</p><p dir="ltr"><b>Classes annotated</b>: Nuclei</p><p dir="ltr"><b>Source (EM) dataset ID</b>: jrc_ctl-id8-1</p><p dir="ltr"><b>Source (EM) voxel size (nm)</b>: 4.00 x 4.00 x 3.48 (X, Y, Z)</p><p dir="ltr"><b>Source (EM) data dimensions (µm)</b>: 74 x 13 x 42 (X, Y, Z)</p><p dir="ltr"><b>Source (EM) DOI</b>: <a href=" https://doi.org/10.25378/janelia.13114454" rel="noreferrer" target="_blank">https://doi.org/10.25378/janelia.13114454</a></p><p dir="ltr"><b>Dataset URL</b>: <a href="https://data.janelia.org/D9CCk" rel="noreferrer" target="_blank">s3://janelia-cosem-datasets/jrc_ctl-id8-1/jrc_ctl-id8-1.zarr/recon-1/labels/inference/segmentations/nuc/</a></p><p dir="ltr"><b>Visualization website</b>: <a href="https://openorganelle.janelia.org/datasets/jrc_ctl-id8-1" rel="noreferrer" target="_blank">https://openorganelle.janelia.org/datasets/jrc_ctl-id8-1</a></p>