Manual nuclei segmentations in near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of Killer T-Cell attacking cancer cell (jrc_ctl-id8-3)

Annotation description: Dense segmentations of nuclei in jrc_ctl-id8-3 using Amira-Avizo (Thermo Fisher Scientific) and "Using Amira to manually segment organelles in vEM for machine learning V.3" protocol.

Annotation protocol: Using Amira to manually segment organelles in vEM for machine learning V.3 (http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3)

Software: Amira 3D 2021.1, Classic Segmentation Workroom

Classes annotated: Nuclei

Source (EM) dataset ID: jrc_ctl-id8-3

Source (EM) voxel size (nm): 8 x 8 x 8 (X, Y, Z)

Source (EM) data dimensions (µm): 70 x 12.8 x 32.24 (X, Y, Z)

Source (EM) DOI: https://doi.org/10.6084/m9.figshare.14447562

Dataset URL: https://data.janelia.org/CQIBE

Visualization website: https://openorganelle.janelia.org/datasets/jrc_ctl-id8-3