Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of Interphase COS-7 cell (jrc_cos7-1b)

Sample: Wild-type, interphase COS-7 cell (ATCC CRL-1651)

Sample Description: A data set featuring isotropic 3D electron microscopy data of a cultured, wild-type, interphase COS-7 cell (ATCC CRL-1651). The sample preparation involved high-pressure freezing, freeze-substitution resin embedding with 2% OsO₄, 0.1% UA, and 3% H₂O in acetone, followed by resin embedding in Eponate 12. Melanie Freeman (Infinimmune) and David Hoffman (10x Genomics) provided the sample, which was prepared for imaging by H. Amalia Pasolli (Rockefeller). The imaging process was conducted by C. Shan Xu (Yale), and post-processing was carried out by Gleb Shtengel and Harald Hess (HHMI/Janelia).

Protocol: High pressure freezing, freeze-substitution resin embedding with 2% OsO₄ 0.1% UA 3% H₂O in acetone; resin embedding in Eponate 12.

Contributions: Sample provided by Melanie Freeman (Infinimmune) and David Hoffman (10x Genomics), prepared for imaging by H. Amalia Pasolli (Rockefeller), imaging by C. Shan Xu (Yale), with post-processing by Gleb Shtengel and Harald Hess(HHMI/Janelia).

Acquisition ID: jrc_cos7-1b

Voxel size (nm): 4 x 4 x 5.08 (x, y, z)

Data dimensions (µm): 22.2 x 6.8 x 2.8 (x, y, z)

Imaging start date: 2016-11-11

Imaging duration (days): 5

Landing energy (eV): 400

Imaging current (nA): 0.25

Scanning speed (MHz): 0.400

Dataset URL (Redirect): https://data.janelia.org/DAk2k

Visualization Website: https://openorganelle.janelia.org/datasets/jrc_cos7-1b

Publication: Xu et al., 2017; Xu et al., 2021