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Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of P7 mouse pancreas (jrc_mus-pancreas-4)
Sample: Pancreas from a postnatal day 7 transgenic mouse with tdTomato-neurons, strain: C57BL/6J from Jackson Lab.
Sample Description: A dataset featuring isotropic 3D electron microscopy data of the mouse pancreas (jrc_mus-pancreas-4). The sample was obtained from a P7, wild-type C57BL/6 mouse strain sourced from Jackson Laboratory. The sample was prepared with perfusion fixation with glutaraldehyde and vibratome section. The region of Interest (ROI) was determined by insulin, glucagon, endogenous neurons multi-labelling. After low temperature reducing OTO staining, the sample was subject to dehydration, infiltration with graded ethanol and Durcupan resin, and polymerized in a 60°C oven. The sample was provided by Wei-Ping Li (HHMI/Janelia), immunofluorescent microscopy by Isabel Espinosa-Medina (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia). Post-processing was conducted by Eric Trautman and Stephan Preibisch (HHMI/Janelia). This dataset offers a comprehensive view of a young mouse pancreas cellular architecture and facilitates further research in digestive and endocrine systems and beyond.
Protocol: Perfusion fixation with glutaraldehyde and vibratome section. Region of Interest (ROI) was determined by insulin, glucagon, endogenous neurons multi-labelling. After low temperature reducing OTO staining, the sample was subject to dehydration, infiltration with graded ethanol and Durcupan resin, and polymerized in a 60°C oven.
Contributions: Sample provided by Wei-Ping Li (HHMI/Janelia), immunofluorescent microscopy by Isabel Espinosa-Medina (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia) and imaged by Wei Qiu (HHMI/Janelia) and post-processing by Eric Trautman and Stephan Preibisch (HHMI/Janelia).
Acquisition ID: jrc_mus-pancreas-4
Voxel size (nm): 4.0 x 4.0 x 4.0 (X, Y, Z)
Data dimensions (µm): 146.272, 113.04 , 115.048 (X, Y, Z)
Imaging start date: 2023-04-18
Dataset URL (Redirect): https://data.janelia.org/5MsI5
Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-pancreas-4
Imaging duration (days): 23
Landing energy (eV):
Imaging current (nA):
Scanning speed (MHz): 3
