Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse cerebellar granule neurons (jrc_mus-granule-neurons-1)

Sample: Cultured mouse cerebellar granule neurons transiently expressing HP1 mEmerald H3.3 SNAP (JF525) Lamin B Halo (JF646)

Sample Description: Near isotropic FIB-SEM imaging of primary mouse granular neurons to study chromatin domains and their reorganization during development. Six cultured cells imaged in this dataset.

Protocol: High pressure freezing, freeze-substitution resin embedding with 2% OsO₄ 0.1% UA 3% H₂O in acetone; resin embedding in Eponate 12.

Contributions: Sample provided by David Solecki (St. Jude Children’s Research Hospital), prepared for imaging by Nirmala Iyer (HHMI/Janelia), with imaging by C. Shan Xu (Yale), and post-processing by Gleb Shtengel, and Harald Hess(HHMI/Janelia).

Acquisition ID: jrc_mus-granule-neurons-1

Voxel size (nm): 4 x 4 x 3.76 (X, Y, Z)

Data dimensions (µm): 52.0 x 5.4 x 16.4 (X, Y, Z)

Imaging start date: 2018-04-27

Imaging duration (days): 11

Landing energy (eV): 1200

Imaging current (nA): 0.14

Scanning speed (MHz): 0.200

Dataset URL (Redirect): https://data.janelia.org/lvev0

Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-granule-neurons-1

Publication: Hoffman et al., 2020; Xu et al., 2017; Xu et al., 2021