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Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse epididymis (jrc_mus-epididymis -1)
Sample: Wild-type, 6 months old mouse, strain: C57BL/6 from Jackson Lab
Sample Description: A dataset featuring isotropic 3D electron microscopy data of the mouse epididymis (jrc_mus-epididymis-2). The sample was obtained from a 6-month-old, wild-type C57BL/6 mouse strain sourced from Jackson Laboratory. Mouse euthanasia was performed as IACUC guidelines followed by immersion fixation in glutaraldehyde and vibratome section. After progressive lowering of temperature dehydration and en bloc staining with heavy metal, the sample was subject to infiltration with acetone and Durcupan resin, and polymerized in 60°C oven. The sample was provided by Zhiyuan Lu (HHMI/Janelia), prepared for imaging by Zhiyuan Lu, and imaged by Wei Qiu (HHMI/Janelia). Post-processing was conducted by Eric Trautman and Stephan Preibisch (HHMI/Janelia). This dataset offers a comprehensive view of the mouse epididymis' cellular architecture and facilitates further research in reproductive biology.
Protocol: Mouse euthanasia was performed as IACUC guidelines followed by immersion fixation in glutaraldehyde and vibratome section. After progressive lowering of temperature dehydration and en bloc staining with heavy metal, the sample was subject to infiltration with acetone and Durcupan resin, and polymerized in 60°C oven.
Contributions: Sample provided by Zhiyuan Lu (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia) and post-processing by Eric Trautman and Stephan Preibisch (HHMI/Janelia).
Acquisition ID: jrc_mus-epididymis-1
Voxel size (nm): 4.0 x 4.0 x 4.0 (X, Y, Z)
Data dimensions (µm): 20.8 x 20.2 x 7.1 (X, Y, Z)
Imaging start date: 2022-07-13
Dataset URL (Redirect): https://data.janelia.org/7hbUt
Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-epididymis-1
Imaging duration (days): 4
Landing energy (eV): 1
Imaging current (nA): .25
Scanning speed (MHz): .200
