Janelia Research Campus
Browse

File(s) stored somewhere else

Please note: Linked content is NOT stored on Janelia Research Campus and we can't guarantee its availability, quality, security or accept any liability.

Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse epididymis (jrc_mus-epididymis -2)

dataset
posted on 2023-06-20, 20:41 authored by CellMap Project TeamCellMap Project Team, Wei-Ping Li, Zhiyuan Lu, Stephan PreibischStephan Preibisch, Wei Qiu, Stephan Saalfeld, Eric T. Trautman

Sample: Wild-type, 6 months old mouse, strain: C57BL/6 from Jackson Lab

Sample Description: A dataset featuring isotropic 3D electron microscopy data of the mouse epididymis (jrc_mus-epididymis-2). The sample was obtained from a 6-month-old, wild-type C57BL/6 mouse strain sourced from Jackson Laboratory. Mouse euthanasia was performed as IACUC guidelines followed by immersion fixation in glutaraldehyde and vibratome section. After room temperature reducing OTO staining, the sample was subject to dehydration, infiltration with graded ethanol and Durcupan resin, and polymerized in a 60°C oven. The sample was provided by Wei-Ping Li (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia). Post-processing was conducted by Eric Trautman and Stephan Preibisch (HHMI/Janelia). This dataset offers a comprehensive view of the mouse epididymis' cellular architecture and facilitates further research in reproductive biology.

Protocol: Mouse euthanasia was performed as IACUC guidelines followed by immersion fixation in glutaraldehyde and vibratome section. After room temperature reducing OTO staining, the sample was subject to dehydration, infiltration with graded ethanol and Durcupan resin, and polymerized in a 60°C oven.

Contributions: Sample provided by Wei-Ping Li and Zhiyuan Lu (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia) and post-processing by Eric Trautman and Stephan Preibisch (HHMI/Janelia).

Acquisition ID: jrc_mus-epididymis -2

Voxel size (nm): 4.0 x 4.0 x 4.0 (X, Y, Z)

Data dimensions (µm): 20.9 x 20.5 x 7.0 (X, Y, Z)

Imaging start date: 2022-07-14

Dataset URL (Redirect): https://data.janelia.org/UXGmn

Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-epididymis-2

Imaging duration (days): 4

Landing energy (eV): 1

Imaging current (nA): 0.25

Scanning speed (MHz): 0.200

History