Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse liver (jrc_mus-liver-2)

Sample: Wild-type, 8 week old mouse, strain: C57BL/6 from Jackson Lab

Sample Description: A dataset featuring isotropic 3D electron microscopy data of the mouse kidney (jrc_mus-kidney-2). The sample was obtained from an 8-week-old, wild-type C57BL/6 mouse strain sourced from Jackson Laboratory. Mouse euthanasia was performed as IACUC guidelines. Pinch out the sample with the biopsy needle followed by high pressure freezing fixation. After low temperature reducing OTO staining, the sample was subject to dehydration, infiltration with graded ethanol and Durcupan resin, and polymerized in a 60°C oven. The sample was provided by Wei-Ping Li (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia). Post-processing was conducted by Eric Trautman and Stephan Preibisch (HHMI/Janelia). This dataset offers a comprehensive view of a young mouse kidney cellular architecture and facilitates further research in hepatology and beyond.

Protocol: Mouse euthanasia was performed as IACUC guidelines. Pinch out the sample with the biopsy needle followed by high pressure freezing fixation. After low temperature reducing OTO staining, the sample was subject to dehydration, infiltration with graded ethanol and Durcupan resin, and polymerized in a 60°C oven.

Contributions: Sample provided by Wei-Ping Li (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), with imaging by Wei Qiu (HHMI/Janelia), and post-processing by Eric Trautman and Stephan Preibisch (HHMI/Janelia).

Acquisition ID: jrc_mus-liver-2

Voxel size (nm): 8.0 x 8.0 x 8.0 (X, Y, Z)

Data dimensions (µm): 82.208, 92.088, 101.704 (X, Y, Z)

Imaging start date: 2022-10-20

Dataset URL (Redirect): https://data.janelia.org/zX4wO

Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-liver-2

Imaging duration (days): 9

Landing energy (eV):

Imaging current (nA):

Scanning speed (MHz): 3.000