Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse nucleus accumbens (jrc_mus-nacc-2)

Sample: Wild-type, adult male mouse, strain: C57/BL6J from Charles River

Sample Description: Exemplary data used to demonstrate the capabilities of Enhanced FIB-SEM systems for large-volume 3D imaging.

Protocol: Chemical fixation, reduced osmium processing, treated with 2% samarium trichloride and 1% uranyl acetate in maleate buffer, then embedded in Durcupan resin.

Contributions: Sample provided by and prepared for imaging by Richard Weinberg (University of North Carolina), with imaging by C. Shan Xu (Yale), Kenneth Hayworth (HHMI/Janelia), and post-processing by Gleb Shtengel, and Harald Hess(HHMI/Janelia).

Acquisition ID: jrc_mus-nacc-2

Voxel size (nm): 4 x 4 x 2.96 (X, Y, Z)

Data dimensions (µm): 10.4 x 10.0 x 1.7 (X, Y, Z)

Imaging start date: 2015-03-09

Imaging duration (days): 2

Landing energy (eV): 200

Imaging current (nA): 0.22

Scanning speed (MHz): 0.200

Dataset URL (Redirect): https://data.janelia.org/PGuRe

Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-nacc-2

Publication: Wu et al., 2017: Xu et al., 2017: Xu et al., 2021