Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse nucleus accumbens (jrc_mus-nacc-4)

Sample: Wild-type, adult male mouse, strain: C57/BL6J from Charles River

Sample Description: Exemplary data used to demonstrate the capabilities of Enhanced FIB-SEM systems for large-volume 3D imaging.

Protocol: Chemical fixation, reduced osmium processing, treated with 2% samarium trichloride and 1% uranyl acetate in maleate buffer, then embedded in Durcupan resin.

Contributions: Sample provided by and prepared for imaging by Richard Weinberg (University of North Carolina), with imaging by C. Shan Xu (Yale), Kenneth Hayworth (HHMI/Janelia), and post-processing by Gleb Shtengel, and Harald Hess(HHMI/Janelia).

Acquisition ID: jrc_mus-nacc-4

Voxel size (nm): 4 x 4 x 3.99 (X, Y, Z)

Data dimensions (µm): 20.3 x 20.5 x 3.0 (X, Y, Z)

Imaging start date: 2014-09-19

Imaging duration (days): 2

Landing energy (eV): 1100

Imaging current (nA): 0.21

Scanning speed (MHz): 0.400

Dataset URL: s3://janelia-cosem-datasets/jrc_mus-nacc-4/jrc_mus-nacc-4.zarr/recon-2/em/

Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-nacc-4

Publication: Wu et al., 2017; Xu et al., 2017; Xu et al., 2021