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Optimization of genetically encoded GABA indicator

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posted on 05.05.2022, 00:29 by Ilya KolbIlya Kolb, Jeremy HassemanJeremy Hasseman, Getahun Tsegaye, Arthur Tsang, Daniel Reep, Jihong ZhengJihong Zheng, Ben ArthurBen Arthur, Jonathan S. Marvin, Karel SvobodaKarel Svoboda, Loren L Looger, Wyatt KorffWyatt Korff, GENIE Project Team

The GENIE Project Team at HHMI Janelia has developed a second-generation genetically encoded GABA indicator (Fig. 1a), in collaboration with Jonathan Marvin and Loren Looger. The sensor is based on iGABASnFR (Marvin, J.S., Shimoda, Y., Magloire, V. et al. A genetically encoded fluorescent sensor for in vivo imaging of GABA. Nat Methods 16, 763–770 (2019). https://doi.org/10.1038/s41592-019-0471-2), and has the following features, tested extensively in cultured neurons.

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