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iGABASnFR2_culture.PNG (475.32 kB)
Optimization of genetically encoded GABA indicator
Version 3 2022-09-15, 21:36
Version 2 2022-05-05, 00:29
Version 1 2022-05-04, 21:24
dataset
posted on 2022-09-15, 21:36 authored by Ilya KolbIlya Kolb, Jeremy HassemanJeremy Hasseman, Getahun Tsegaye, Arthur Tsang, Daniel Reep, Jihong ZhengJihong Zheng, Ben ArthurBen Arthur, Jonathan S. Marvin, Karel SvobodaKarel Svoboda, Loren L Looger, Wyatt KorffWyatt Korff, GENIE Project Team
The GENIE Project Team at HHMI Janelia has developed a second-generation genetically encoded GABA indicator, in collaboration with Jonathan Marvin and Loren Looger. The sensor is based on iGABASnFR (1), and has the following features, tested extensively in cultured neurons:
- Positive-going version (iGABASnFR2) and negative-going version (iGABASnFR2n)
- iGABASnFR2: 4x higher sensitivity to APs than iGABASnFR, iGABASnFR2n: 2x higher sensitivity to APs than iGABASnFR.
- Both enable single-AP high-speed imaging
- Enables detection of signal in individual dendrites and synaptic boutons
- Higher affinity for GABA (iGABASnFR2: EC50: 6.4±0.2 μM, ~7x tighter than iGABASnFR, ΔF/Fmax=0.5±0.05, ~2x higher than iGABASnFR)
- Imaged with standard GFP filter set
