Refined, automatic nuclei segmentations in near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of isolated murine pancreatic islets (jrc_mus-pancreas-1)

Segmentation description: Nuclei segmentations in jrc_mus-pancreas-1 using Cellpose 3.0.9 with cursory manual corrections.

Software: Cellpose 3.0.9

Software DOI: https://doi.org/10.1038/s41592-022-01663-4

Prediction Duration (seconds): 599.51

GPU: NVIDIA TITAN RTX

RAM: 251.0 GiB

Label voxel size (nm): 128 x 128 x 128 (x, y, z)

Reconstructed data dimensions (µm): 30 x 20 x 25 (x, y, z)

Classes annotated: Nucleus

Source dataset (EM) ID: jrc_mus-pancreas-1

Source dataset (EM) voxel size (nm): 4.0 x 4.0 x 3.4 (x, y, z)

Source dataset (EM) data dimensions (µm): 30 x 20 x 25 (x, y, z)

Source dataset (EM) DOI: https://doi.org/10.25378/janelia.13114499

Dataset URL: s3://janelia-cosem-datasets/jrc_mus-pancreas-1/jrc_mus-pancreas-1.zarr/recon-1/labels/inference/segmentations/nuc/

Visualization website: https://openorganelle.janelia.org/datasets/jrc_mus-pancreas-1