jGC8_TransgenicMice_WhitePaper_v3.pdf (3.25 MB)
jGCaMP8 transgenic mice
dataset
posted on 2023-11-15, 14:16 authored by Tim Wang, Marius PachitariuMarius Pachitariu, Caiying Guo, Albert K Lee, Jae Sung Lee, Yan Zhang, Marton RozsaMarton Rozsa, Jeremy HassemanJeremy Hasseman, Ilya KolbIlya Kolb, Jim D. Cox, Crystal Lopez, Loren Looger, Karel SvobodaKarel SvobodaThe GENIE Project Team at HHMI Janelia Research Campus have developed and characterized multiple transgenic mice expressing jGCaMP8s and jGCaMP8m and deposited the lines at The Jackson Laboratory (JAX). jGCaMP8s and jGCaMP8m are genetically encoded calcium indicators with fast kinetics (~5 fold shorter rise time) than jGCaMP7f and high sensitivity (2X higher 1AP SNR) than jGCaMP7s (see https://doi.org/10.1038/s41586-023-05828-9 for details). This white paper provides updates on developments in each transgenic mouse line and how to obtain them.
The novel mouse lines include:
1) Tetracycline‐controlled transactivator protein (tTA)-dependent expression
- JAX 037717 TetO‐jGCaMP8s
2) Cre recombinase-dependent expression
- JAX 037718 TIGRE2‐jGCaMP8m‐IRES‐tTA2‐WPRE
- JAX 037719 TIGRE2‐jGCaMP8s‐IRES‐tTA2
- JAX 037952 TIGRE2-jGCaMP8s-IRES-tTA2-WPRE
- TIGRE2‐RiboL1-jGCaMP8s‐IRES‐tTA2 (JAX deposit pending)
