<p dir="ltr">High-throughput imaging of <i>Drosophila</i> central nervous system specimens requires precise, standardized arrangement of the samples on a microscope slide. A simple grid makes locating the specimens a straightforward task and streamlines the imaging process. A standardized system of tissue arrangement also guarantees repeatable identification of individual samples.<br><br>The <i>Drosophila</i> Mounting T-Dish is a simple device which provides a template for mounting dissected fly nervous systems in a regular array on a glass coverslip. In this device, the coverslip rests on an etched grid of T-shaped marks at the bottom of a saline-filled well. The etched marks help the user place the specimens in standardized positions that allow the imager to quickly locate samples of interest. The marks also help arrange the tissue in the proper orientation to fit an entire adult fly brain into a single 20X microscope field. The mounting dish includes wells to hold specimens in saline until they are mounted and cutouts to ease handling of the coverslip.<br><br>The standardized arrangement and orientation of <i>Drosophila</i> nervous systems substantially shortens the time required to acquire high-resolution images. In combination with a slide holder that provides repeatable slide positioning, such as the JFRC Quick-Mount Slide Holder, the T-dish makes it possible to correctly identify individual specimens during multiple imaging sessions. The consistent orientation also minimizes the number of images that must be acquired to document an entire nervous system, further reducing the imaging time and the accompanying costs.<br><br>The <i>Drosophila</i> Mounting T-dish is designed to mount 80 nervous systems on a 22x22mm coverslip, but modifications for different numbers of specimens and various coverslip sizes are possible.<br><br><b>Advantages:</b></p><ul><li>Simplifies the regular arrangement of <i>Drosophila</i> CNS samples on a microscope coverslip.</li><li>Improves imaging efficiency.</li><li>Allows reliable identification of individual specimens.</li></ul><p dir="ltr"><b>Applications:</b></p><ul><li>Compatible with many standard imaging techniques, from low-resolution widefield microscopy to high-resolution confocal microscopy.</li><li>Useful in high-throughput, automated microscopy of insect nervous systems or any specimens that benefit from standardized arrangement on a slide.</li></ul><p dir="ltr"><b>Use</b></p><p dir="ltr">Free to make for Non-Profit Research by downloading designs at GitHub link.<b><u>Please cite this DOI.</u></b><br>Commercial licenses are also available, contact innovation@janelia.hhmi.org and reference this DOI.</p><p><br></p><p dir="ltr"><b>Other</b></p><p dir="ltr"><i>Supersedes Flintbox ID: Janelia</i><i> </i><i>2014-037</i><br></p>
